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ATCC homo sapiens bone osteosarcoma u2os cells

( A ) A schematic illustrates the process of LatA treatment assay in <t>U2OS</t> cell lines on nanostructures. ( B ) Average confocal images of LifeAct-mApple-expressed U2OS live cells cultured on nanobars ranging from 300 to 1000 nm in width demonstrate F-actin recovery following a 1 h treatment with 100 nM LatA and a 20 min washout. Scale bar, 2 μm. ( C ) Normalized actin signal density was calculated based on the actin intensity in the flat area, at nanobars ranging from 300–1000 nm in width. Sample sizes for treatments under DMSO, LatA and washout were N = 6, 6, and 8 averaged nanobar ends, respectively. Each data point represents the mean ± SEM. ( D ) The normalized actin repolymerization ratio (Washout/LatA) in ( C ) at nanobars ranging from 300–1000 nm in width. N = 8 averaged nanobars, each point represents mean ± SEM. ( E ) Original and averaged confocal images of U2OS fixed cells cultured on 300-nm-wide nanobars with the expression of mScarlet3-N-WASP and the staining with Phalloidin-ATTO-488. Cells were fixed after a 1 h treatment with DMSO, 20 or 50 μM ML141. Scale bar, 10 μm (left) and 1 μm (right). Contrast(averaged): 12–30. ( F ) Actin signal end-to-center ratio at nanobars 300 nm in width. Sample sizes for treatments under DMSO, 20 or 50 µM ML141 were N = 634, 324, 689 nanobar ends, respectively. Each data point represents the mean ± SD. (**** P < 0.0001). ( G ) mScarlet-N-WASP signal end-to-center ratio at nanobars 300 nm in width. Sample sizes for treatments under DMSO, 20 or 50 µM ML141 were N = 634, 324, 689 nanobar ends, respectively. Each data point represents the mean ± SD. ( H ) Average confocal images of U2OS cells cultured on nanobar arrays with bar widths ranging from 1000 to 300 nm, immuno-stained with anti-FBP17. Cells were treated with DMSO, 20 μM ML141, or 50 μM ML141. Scale bars: 2 μm. ( I ) Quantification of FBP17 intensity at nanobar ends across bar widths for each treatment condition in ( H ). Data represent mean ± SEM from N = 45–57 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). ( J ) Average confocal images of U2OS cells cultured on nanobar arrays with bar widths ranging from 1000 to 300 nm, stained with phalloidin-565. Cells were treated with DMSO, 20 μM ML141, or 50 μM ML141. Scale bars: 2 μm. ( K ) Quantification of Phalloidin-565(F-actin) intensity at nanobar ends across bar widths for each treatment condition in ( J ). Data represent mean ± SEM from N = 34–54 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. See also Fig. . .

Homo Sapiens Bone Osteosarcoma U2os Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Membrane curvature initiates Cdc42-FBP17-N-WASP clustering and actin nucleation"

Article Title: Membrane curvature initiates Cdc42-FBP17-N-WASP clustering and actin nucleation

Journal: The EMBO Journal

doi: 10.1038/s44318-025-00677-w

( A ) A schematic illustrates the process of LatA treatment assay in U2OS cell lines on nanostructures. ( B ) Average confocal images of LifeAct-mApple-expressed U2OS live cells cultured on nanobars ranging from 300 to 1000 nm in width demonstrate F-actin recovery following a 1 h treatment with 100 nM LatA and a 20 min washout. Scale bar, 2 μm. ( C ) Normalized actin signal density was calculated based on the actin intensity in the flat area, at nanobars ranging from 300–1000 nm in width. Sample sizes for treatments under DMSO, LatA and washout were N = 6, 6, and 8 averaged nanobar ends, respectively. Each data point represents the mean ± SEM. ( D ) The normalized actin repolymerization ratio (Washout/LatA) in ( C ) at nanobars ranging from 300–1000 nm in width. N = 8 averaged nanobars, each point represents mean ± SEM. ( E ) Original and averaged confocal images of U2OS fixed cells cultured on 300-nm-wide nanobars with the expression of mScarlet3-N-WASP and the staining with Phalloidin-ATTO-488. Cells were fixed after a 1 h treatment with DMSO, 20 or 50 μM ML141. Scale bar, 10 μm (left) and 1 μm (right). Contrast(averaged): 12–30. ( F ) Actin signal end-to-center ratio at nanobars 300 nm in width. Sample sizes for treatments under DMSO, 20 or 50 µM ML141 were N = 634, 324, 689 nanobar ends, respectively. Each data point represents the mean ± SD. (**** P < 0.0001). ( G ) mScarlet-N-WASP signal end-to-center ratio at nanobars 300 nm in width. Sample sizes for treatments under DMSO, 20 or 50 µM ML141 were N = 634, 324, 689 nanobar ends, respectively. Each data point represents the mean ± SD. ( H ) Average confocal images of U2OS cells cultured on nanobar arrays with bar widths ranging from 1000 to 300 nm, immuno-stained with anti-FBP17. Cells were treated with DMSO, 20 μM ML141, or 50 μM ML141. Scale bars: 2 μm. ( I ) Quantification of FBP17 intensity at nanobar ends across bar widths for each treatment condition in ( H ). Data represent mean ± SEM from N = 45–57 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). ( J ) Average confocal images of U2OS cells cultured on nanobar arrays with bar widths ranging from 1000 to 300 nm, stained with phalloidin-565. Cells were treated with DMSO, 20 μM ML141, or 50 μM ML141. Scale bars: 2 μm. ( K ) Quantification of Phalloidin-565(F-actin) intensity at nanobar ends across bar widths for each treatment condition in ( J ). Data represent mean ± SEM from N = 34–54 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. See also Fig. . .

Figure Legend Snippet: ( A ) A schematic illustrates the process of LatA treatment assay in U2OS cell lines on nanostructures. ( B ) Average confocal images of LifeAct-mApple-expressed U2OS live cells cultured on nanobars ranging from 300 to 1000 nm in width demonstrate F-actin recovery following a 1 h treatment with 100 nM LatA and a 20 min washout. Scale bar, 2 μm. ( C ) Normalized actin signal density was calculated based on the actin intensity in the flat area, at nanobars ranging from 300–1000 nm in width. Sample sizes for treatments under DMSO, LatA and washout were N = 6, 6, and 8 averaged nanobar ends, respectively. Each data point represents the mean ± SEM. ( D ) The normalized actin repolymerization ratio (Washout/LatA) in ( C ) at nanobars ranging from 300–1000 nm in width. N = 8 averaged nanobars, each point represents mean ± SEM. ( E ) Original and averaged confocal images of U2OS fixed cells cultured on 300-nm-wide nanobars with the expression of mScarlet3-N-WASP and the staining with Phalloidin-ATTO-488. Cells were fixed after a 1 h treatment with DMSO, 20 or 50 μM ML141. Scale bar, 10 μm (left) and 1 μm (right). Contrast(averaged): 12–30. ( F ) Actin signal end-to-center ratio at nanobars 300 nm in width. Sample sizes for treatments under DMSO, 20 or 50 µM ML141 were N = 634, 324, 689 nanobar ends, respectively. Each data point represents the mean ± SD. (**** P < 0.0001). ( G ) mScarlet-N-WASP signal end-to-center ratio at nanobars 300 nm in width. Sample sizes for treatments under DMSO, 20 or 50 µM ML141 were N = 634, 324, 689 nanobar ends, respectively. Each data point represents the mean ± SD. ( H ) Average confocal images of U2OS cells cultured on nanobar arrays with bar widths ranging from 1000 to 300 nm, immuno-stained with anti-FBP17. Cells were treated with DMSO, 20 μM ML141, or 50 μM ML141. Scale bars: 2 μm. ( I ) Quantification of FBP17 intensity at nanobar ends across bar widths for each treatment condition in ( H ). Data represent mean ± SEM from N = 45–57 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). ( J ) Average confocal images of U2OS cells cultured on nanobar arrays with bar widths ranging from 1000 to 300 nm, stained with phalloidin-565. Cells were treated with DMSO, 20 μM ML141, or 50 μM ML141. Scale bars: 2 μm. ( K ) Quantification of Phalloidin-565(F-actin) intensity at nanobar ends across bar widths for each treatment condition in ( J ). Data represent mean ± SEM from N = 34–54 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. See also Fig. . .

Techniques Used: Cell Culture, Expressing, Staining

( A ) Representative confocal images of Lifeact-mApple-labeled actin filaments in cells treated with DMSO (control), LatA, or after LatA washout, cultured on flat surfaces (top row) or nanobar arrays (bottom row). White boxes in nanobar images indicate regions of interest (ROIs) corresponding to bar widths ranging from 300 to 1000 nm. Scale bars: 10 µm. Zoomed-in views of nanobar ROIs, highlighting actin alignment and curvature adaptation across different bar widths (300–1000 nm). ( B ) Original and averaged confocal images of U2OS fixed cells cultured on 300-nm-wide nanobars with the expression of mScarlet3-N-WASP and the staining with Phalloidin-ATTO-488. Cells were fixed after a 1 h treatment with 40 μM CK666. Scale bar, 10 μm (left) and 1 μm (right). Contrast(averaged): 12–30. ( C ) Normalized Actin signal end-to-center ratio at nanobars 300 nm in width. Sample sizes for treatments under DMSO, 20 or 50 µM ML141, 40 µM CK666 were N = 634, 324, 689, and 425 nanobar ends, respectively. Each data point represents the mean ± SEM. ( D ) Normalized mScarlet-N-WASP signal end-to-center ratio at nanobars 300 nm in width. Sample sizes for treatments under DMSO, 20 or 50 µM ML141, 40 µM CK666 were N = 634, 324, 689, and 425 nanobar ends, respectively. Each data point represents the mean ± SEM. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test (ns p > 0.05, **** p < 0.0001).

Figure Legend Snippet: ( A ) Representative confocal images of Lifeact-mApple-labeled actin filaments in cells treated with DMSO (control), LatA, or after LatA washout, cultured on flat surfaces (top row) or nanobar arrays (bottom row). White boxes in nanobar images indicate regions of interest (ROIs) corresponding to bar widths ranging from 300 to 1000 nm. Scale bars: 10 µm. Zoomed-in views of nanobar ROIs, highlighting actin alignment and curvature adaptation across different bar widths (300–1000 nm). ( B ) Original and averaged confocal images of U2OS fixed cells cultured on 300-nm-wide nanobars with the expression of mScarlet3-N-WASP and the staining with Phalloidin-ATTO-488. Cells were fixed after a 1 h treatment with 40 μM CK666. Scale bar, 10 μm (left) and 1 μm (right). Contrast(averaged): 12–30. ( C ) Normalized Actin signal end-to-center ratio at nanobars 300 nm in width. Sample sizes for treatments under DMSO, 20 or 50 µM ML141, 40 µM CK666 were N = 634, 324, 689, and 425 nanobar ends, respectively. Each data point represents the mean ± SEM. ( D ) Normalized mScarlet-N-WASP signal end-to-center ratio at nanobars 300 nm in width. Sample sizes for treatments under DMSO, 20 or 50 µM ML141, 40 µM CK666 were N = 634, 324, 689, and 425 nanobar ends, respectively. Each data point represents the mean ± SEM. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test (ns p > 0.05, **** p < 0.0001).

Techniques Used: Labeling, Control, Cell Culture, Expressing, Staining

( A ) Model of N-WASP activation by FBP17 (upper) and Cdc42 binding (lower). ( B ) Domain organization of FBP17, the HR1-deleted mutant (FdHR1), and the tip-to-tip oligomerization-deficient mutant (K166A). ( C ) Schematic and representative single-molecule imaging of FBP17 and K166A clustering (10% AF647 labeled). Scale bars, 1 μm. ( D ) Quantification of total intensity for FBP17 and K166A clusters. ( E ) Frequency distribution of total intensity for FBP17 and K166A clusters. ( F ) Average confocal images of U2OS cells seeded on nanobar arrays, expressing FBP17, FdHR1, or K166A. Scale bars, 2 μm. ( G ) Average confocal images of N-WASP recruitment in U2OS cells expressing FBP17, FdHR1, or K166A. Scale bars, 2 μm. ( H ) Average confocal images of phalloidin staining (F-actin) in U2OS cells expressing N-WASP with FBP17, FdHR1, or K166A. Scale bars, 2 μm. ( I ) Quantification of nanobar end-to-center ratio for FBP17, FdHR1, and K166A in ( F ). Data represent mean ± SEM from N = 22–64 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). ( J ) Quantification of nanobar end-to-center ratio for N-WASP in ( G ). Data represent mean ± SEM from N = 23–64 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). ( K ) Quantification of nanobar end-to-center ratio for phalloidin in ( H ). Data represent mean ± SEM from N = 20–70 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). Statistical analysis was performed using unpaired t -test or two-way ANOVA followed by Tukey’s multiple comparisons test. Outliers were identified and excluded using the ROUT method ( Q = 1%). .

Figure Legend Snippet: ( A ) Model of N-WASP activation by FBP17 (upper) and Cdc42 binding (lower). ( B ) Domain organization of FBP17, the HR1-deleted mutant (FdHR1), and the tip-to-tip oligomerization-deficient mutant (K166A). ( C ) Schematic and representative single-molecule imaging of FBP17 and K166A clustering (10% AF647 labeled). Scale bars, 1 μm. ( D ) Quantification of total intensity for FBP17 and K166A clusters. ( E ) Frequency distribution of total intensity for FBP17 and K166A clusters. ( F ) Average confocal images of U2OS cells seeded on nanobar arrays, expressing FBP17, FdHR1, or K166A. Scale bars, 2 μm. ( G ) Average confocal images of N-WASP recruitment in U2OS cells expressing FBP17, FdHR1, or K166A. Scale bars, 2 μm. ( H ) Average confocal images of phalloidin staining (F-actin) in U2OS cells expressing N-WASP with FBP17, FdHR1, or K166A. Scale bars, 2 μm. ( I ) Quantification of nanobar end-to-center ratio for FBP17, FdHR1, and K166A in ( F ). Data represent mean ± SEM from N = 22–64 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). ( J ) Quantification of nanobar end-to-center ratio for N-WASP in ( G ). Data represent mean ± SEM from N = 23–64 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). ( K ) Quantification of nanobar end-to-center ratio for phalloidin in ( H ). Data represent mean ± SEM from N = 20–70 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). Statistical analysis was performed using unpaired t -test or two-way ANOVA followed by Tukey’s multiple comparisons test. Outliers were identified and excluded using the ROUT method ( Q = 1%). .

Techniques Used: Activation Assay, Binding Assay, Mutagenesis, Imaging, Labeling, Expressing, Staining

Battery:

Article Title: Tear-Based Battery Embedded with Prussian Blue Analogues and Silver Nanoparticles for Smart Contact Lenses.
Article Snippet: Smart contact lenses coupled with electronic components have vast potential for healthcare applications, as such advanced technology enables continuous and noninvasive monitoring of vital signs and disease diagnosis.. However, stringent safety and nontoxicity requirements for ocular devices restrict the applications of flexible batteries fabricated by conventional methods.. Here, we design a safe and flexible tearbased aqueous battery composed of Prussian blue analogues and silver nanoparticle films for cathode and anode, respectively.

Cell Culture:

Article Title: Patterning of Oncogenic Ras Clustering in Live Cells Using Vertically Aligned Nanostructure Arrays.
Article Snippet: Fibronectin (2 μg/mL; SigmaAldrich) was added for coating for 30 min at 37 °C in order to promote cell attachment. .. Homo sapiens bone osteosarcoma U2OS cells (ATCC) were then cultured onto the substrate one day before transfection. .. DMEM with GlutaMAX (Gibco) with 10% fetal bovine serum (FBS, Life Technologies) and 1% Penicillin-Streptomycin (Life Technologies) was used for cell growth in a standard incubator with a temperature of 37 °C and 5% CO2.

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Journal: The EMBO Journal

Article Title: Membrane curvature initiates Cdc42-FBP17-N-WASP clustering and actin nucleation

doi: 10.1038/s44318-025-00677-w

Figure Lengend Snippet: ( A ) A schematic illustrates the process of LatA treatment assay in U2OS cell lines on nanostructures. ( B ) Average confocal images of LifeAct-mApple-expressed U2OS live cells cultured on nanobars ranging from 300 to 1000 nm in width demonstrate F-actin recovery following a 1 h treatment with 100 nM LatA and a 20 min washout. Scale bar, 2 μm. ( C ) Normalized actin signal density was calculated based on the actin intensity in the flat area, at nanobars ranging from 300–1000 nm in width. Sample sizes for treatments under DMSO, LatA and washout were N = 6, 6, and 8 averaged nanobar ends, respectively. Each data point represents the mean ± SEM. ( D ) The normalized actin repolymerization ratio (Washout/LatA) in ( C ) at nanobars ranging from 300–1000 nm in width. N = 8 averaged nanobars, each point represents mean ± SEM. ( E ) Original and averaged confocal images of U2OS fixed cells cultured on 300-nm-wide nanobars with the expression of mScarlet3-N-WASP and the staining with Phalloidin-ATTO-488. Cells were fixed after a 1 h treatment with DMSO, 20 or 50 μM ML141. Scale bar, 10 μm (left) and 1 μm (right). Contrast(averaged): 12–30. ( F ) Actin signal end-to-center ratio at nanobars 300 nm in width. Sample sizes for treatments under DMSO, 20 or 50 µM ML141 were N = 634, 324, 689 nanobar ends, respectively. Each data point represents the mean ± SD. (**** P < 0.0001). ( G ) mScarlet-N-WASP signal end-to-center ratio at nanobars 300 nm in width. Sample sizes for treatments under DMSO, 20 or 50 µM ML141 were N = 634, 324, 689 nanobar ends, respectively. Each data point represents the mean ± SD. ( H ) Average confocal images of U2OS cells cultured on nanobar arrays with bar widths ranging from 1000 to 300 nm, immuno-stained with anti-FBP17. Cells were treated with DMSO, 20 μM ML141, or 50 μM ML141. Scale bars: 2 μm. ( I ) Quantification of FBP17 intensity at nanobar ends across bar widths for each treatment condition in ( H ). Data represent mean ± SEM from N = 45–57 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). ( J ) Average confocal images of U2OS cells cultured on nanobar arrays with bar widths ranging from 1000 to 300 nm, stained with phalloidin-565. Cells were treated with DMSO, 20 μM ML141, or 50 μM ML141. Scale bars: 2 μm. ( K ) Quantification of Phalloidin-565(F-actin) intensity at nanobar ends across bar widths for each treatment condition in ( J ). Data represent mean ± SEM from N = 34–54 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test. See also Fig. . .

Article Snippet: Homo sapiens bone osteosarcoma U2OS cells (ATCC) were maintained in DMEM with GlutaMAX (Gibco) supplemented medium with 10% fetal bovine serum (FBS) and 1% Penicillin/Streptomycin (PS).

Techniques: Cell Culture, Expressing, Staining

Journal: The EMBO Journal

Article Title: Membrane curvature initiates Cdc42-FBP17-N-WASP clustering and actin nucleation

doi: 10.1038/s44318-025-00677-w

Figure Lengend Snippet: ( A ) Representative confocal images of Lifeact-mApple-labeled actin filaments in cells treated with DMSO (control), LatA, or after LatA washout, cultured on flat surfaces (top row) or nanobar arrays (bottom row). White boxes in nanobar images indicate regions of interest (ROIs) corresponding to bar widths ranging from 300 to 1000 nm. Scale bars: 10 µm. Zoomed-in views of nanobar ROIs, highlighting actin alignment and curvature adaptation across different bar widths (300–1000 nm). ( B ) Original and averaged confocal images of U2OS fixed cells cultured on 300-nm-wide nanobars with the expression of mScarlet3-N-WASP and the staining with Phalloidin-ATTO-488. Cells were fixed after a 1 h treatment with 40 μM CK666. Scale bar, 10 μm (left) and 1 μm (right). Contrast(averaged): 12–30. ( C ) Normalized Actin signal end-to-center ratio at nanobars 300 nm in width. Sample sizes for treatments under DMSO, 20 or 50 µM ML141, 40 µM CK666 were N = 634, 324, 689, and 425 nanobar ends, respectively. Each data point represents the mean ± SEM. ( D ) Normalized mScarlet-N-WASP signal end-to-center ratio at nanobars 300 nm in width. Sample sizes for treatments under DMSO, 20 or 50 µM ML141, 40 µM CK666 were N = 634, 324, 689, and 425 nanobar ends, respectively. Each data point represents the mean ± SEM. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparisons test (ns p > 0.05, **** p < 0.0001).

Techniques: Labeling, Control, Cell Culture, Expressing, Staining

Journal: The EMBO Journal

Article Title: Membrane curvature initiates Cdc42-FBP17-N-WASP clustering and actin nucleation

doi: 10.1038/s44318-025-00677-w

Figure Lengend Snippet: ( A ) Model of N-WASP activation by FBP17 (upper) and Cdc42 binding (lower). ( B ) Domain organization of FBP17, the HR1-deleted mutant (FdHR1), and the tip-to-tip oligomerization-deficient mutant (K166A). ( C ) Schematic and representative single-molecule imaging of FBP17 and K166A clustering (10% AF647 labeled). Scale bars, 1 μm. ( D ) Quantification of total intensity for FBP17 and K166A clusters. ( E ) Frequency distribution of total intensity for FBP17 and K166A clusters. ( F ) Average confocal images of U2OS cells seeded on nanobar arrays, expressing FBP17, FdHR1, or K166A. Scale bars, 2 μm. ( G ) Average confocal images of N-WASP recruitment in U2OS cells expressing FBP17, FdHR1, or K166A. Scale bars, 2 μm. ( H ) Average confocal images of phalloidin staining (F-actin) in U2OS cells expressing N-WASP with FBP17, FdHR1, or K166A. Scale bars, 2 μm. ( I ) Quantification of nanobar end-to-center ratio for FBP17, FdHR1, and K166A in ( F ). Data represent mean ± SEM from N = 22–64 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). ( J ) Quantification of nanobar end-to-center ratio for N-WASP in ( G ). Data represent mean ± SEM from N = 23–64 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). ( K ) Quantification of nanobar end-to-center ratio for phalloidin in ( H ). Data represent mean ± SEM from N = 20–70 averaged nanobar ends per bar width per condition (exact n values are provided in the Source Data). Statistical analysis was performed using unpaired t -test or two-way ANOVA followed by Tukey’s multiple comparisons test. Outliers were identified and excluded using the ROUT method ( Q = 1%). .

Techniques: Activation Assay, Binding Assay, Mutagenesis, Imaging, Labeling, Expressing, Staining

A. A schematic illustrates the process of in vivo LatA treatment assay in U2OS cell lines on nanostructures. B. Averaged confocal images of LifeAct-mApple-expressed U2OS live cells cultured on nanobars ranging from 300 to 1000 nm in width demonstrate F-actin recovery following a 1h treatment with 100 nM LatA and a 20min washout. Scale bar, 2 μm. C. Normalized actin signal density calculated based on the actin intensity in the flat area, at nanobars ranging from 300-1000 nm in width. Each point represents mean ± SEM from over 50 nanobar ends. D. The normalized actin repolymerization ratio (Washout / LatA) in (C) at nanobars ranging from 300-1000 nm in width. Each point represents mean ± SEM from over 20 nanobar ends. E. A schematic illustrates the process of in vitro reconstitution of FBP17-mediated actin polymerization at membrane curvatures. F. Averaged confocal images of in vitro reconstitution of 1.5 μM actin (10% Oregon labeled) polymerization at 0/90/180s on the bilayer at nanobar of 200-1000 nm width with the presence of 200 nM FBP17, 50 nM N-WASP, 12 nM CapZ and 5 nM Arp2/3. Scale bar, 2 μm. See also Movie S1. G. Normalized actin signal intensity based on their corresponding lipid bilayer intensity at nanobars of 200-1000 nm width. Each point represents mean ± SEM from 4 nanobars. H. Actin polymerization rate at nanobars of 200-1000 nm width. (The linear fitted slope within 105-350s range in G). Each point represents mean ± SD, N=4. See also Supplementary Figure S3.

Journal: bioRxiv

Article Title: Membrane curvature catalyzes actin nucleation through nano-scale condensation of N-WASP-FBP17

doi: 10.1101/2024.04.25.591054

Figure Lengend Snippet: A. A schematic illustrates the process of in vivo LatA treatment assay in U2OS cell lines on nanostructures. B. Averaged confocal images of LifeAct-mApple-expressed U2OS live cells cultured on nanobars ranging from 300 to 1000 nm in width demonstrate F-actin recovery following a 1h treatment with 100 nM LatA and a 20min washout. Scale bar, 2 μm. C. Normalized actin signal density calculated based on the actin intensity in the flat area, at nanobars ranging from 300-1000 nm in width. Each point represents mean ± SEM from over 50 nanobar ends. D. The normalized actin repolymerization ratio (Washout / LatA) in (C) at nanobars ranging from 300-1000 nm in width. Each point represents mean ± SEM from over 20 nanobar ends. E. A schematic illustrates the process of in vitro reconstitution of FBP17-mediated actin polymerization at membrane curvatures. F. Averaged confocal images of in vitro reconstitution of 1.5 μM actin (10% Oregon labeled) polymerization at 0/90/180s on the bilayer at nanobar of 200-1000 nm width with the presence of 200 nM FBP17, 50 nM N-WASP, 12 nM CapZ and 5 nM Arp2/3. Scale bar, 2 μm. See also Movie S1. G. Normalized actin signal intensity based on their corresponding lipid bilayer intensity at nanobars of 200-1000 nm width. Each point represents mean ± SEM from 4 nanobars. H. Actin polymerization rate at nanobars of 200-1000 nm width. (The linear fitted slope within 105-350s range in G). Each point represents mean ± SD, N=4. See also Supplementary Figure S3.

Article Snippet: Homosapiens bone osteosarcoma U2OS cells (ATCC) were maintained in DMEM with GlutaMAX (Gibco) supplemented medium with 10% FBS. and 1% PS.

Techniques: In Vivo, Cell Culture, In Vitro, Membrane, Labeling

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homo sapiens bone osteosarcoma u2os cells (ATCC)

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